Time Request Materials

This page details the information you will be asked to provide when completing a microscope time request on the User Portal.

Project Information

  • Project Number
  • Project Title
  • Principle Investigator
  • Institution
  • Citizenship
  • Phone
  • Email
  • Lead Contact
  • Lead Contact’s Email
  • Lead Contact’s Phone

Methods & Equipment Information

What technique do you want to perform? (Pick only one)

  • Single Particle Analysis (SPA)
  • Tomography
  • Correlative Fluorescent Microscopy & Tomography
  • Diffraction
  • Other

What type of instrument(s) are you planning to use? (Select all that apply)

  • Microscope
    • 300 kV or 200 kV
    • With or without energy filter
    • With or without phase plate
  • Freezing Apparatus
    • Vitrobot
    • Vitrojet
    • Chameleon
  • Cryo-CLEM
  • Cryo-FIB
  • Tissue culture lab
Is there any foreign funding supporting this project? (Y/N)

Sample Information

Biological Material? (Y/N)

1) List all samples
List EACH biological sample specifically.  Generalized descriptions such as "membrane proteins" and "E. Coli" are too vague and unacceptable.  Specify the biosafety level (BSL) of each sample and provide an IBC or APB protocol number, if BSL2.  Samples are described in further detail in subsections 3-9, as appropriate.
2) IBC Approval
Provide IBC documentation (IBC-approved protocol or a letter from IBC or Biosafety Officer) for all RG2 agents.  if a listed RG2 agent is inactivated, confirmation that the method of inactivation is sufficient must be provided.  The samples will not be approved to be received, manipulated, or handled at SLAC without the IBC documents.
3) Bacteria, fungi, parasites
Describe the BSL of the agent, source, strain, catalog number, and permits from regulatory agencies.  If the agent is registered with the IBC, provide the information in the IBC Approval section (subsection 2).  Enter N/A, if not applicable to your sample(s).
4) Cell/Cell line
Describe the source and any manipulation done.  if the cells were genetically manipulated, describe the details the vector used, transgene expressed, and method of inactivation.  Enter N/A, if not applicable to your sample(s).
5) Virus or viral components
Describe the strain, source, method of expression or purification, and method of inactivation.  If the agent is registered with the IBC, provide the information in the IBC Approval section (section 2).  Please see Common Viruses and Viral Vectors employed in molecular biology at Stanford/SLAC for further details.  Enter N/A, if not applicable to your sample(s).
6) Prion-like protein
Describe any known function, any known toxicity/hazards, and the source organism.  Please see Stanford's Requirements for Research Involving Prion-like Proteins for further details.  Enter N/A, if not applicable to your sample(s).
7) Select Agents and Toxins
Describe the select agent or toxin.  These are regulated biological agents and toxins that have been determined to have the potential to pose a severe threat to both human and animal health, to plant health, or to animal and plant products by the U.S. Department of Health and Human Services (HHS) or the U.S. Department of Agriculture (USDA).  Please see the Select Agents and Toxins List for a comprehensive list.  Enter N/A, if not applicable to your sample(s).
8) Protein
Describe the expression method/system, vectors/cell line used, purification method, and inactivation method.  If a viral vector is used, describe it in the virus or viral components section (subsection 5).  Enter N/A, if not applicable to your sample(s).
9) Nucleic acids
Describe the general properties, source, source agent, and the NIH Guidelines category.  Please see the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules for further details.  Enter N/A, if not applicable to your sample(s).

Supporting or Preliminary Data

CryoEM Image, Slice, or Tomogram (pdf, if available)

Scheduling

  • Optimal number of days
  • Minimal number of days
  • Dates when you absolutely cannot accept microscope time (i.e., scheduling conflicts)
  • Other comments relevant to scheduling
  • Indicate your preferred experiment dates (mm/dd/yyyy)

Research Team & Collaborators

Remove any team members not coming, either onsite or remotely, to the requested microscope session, identify those who are participating onsite or remotely, and authorize team member access to experiment data, as needed.

Shipping

At the conclusion of your experiment, do you plan to ship samples, equipment, or other scientific items from SLAC to an international location? (Y/N)

 

Last Updated

Wed, 09/14/2022 - 08:39