Frequently Asked Questions (FAQs)
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When my project expires can I request an extension?
Answer:
Yes, upon submitting an extension form you may have a one-year extension. Pls should contact our S2C2 User Administrator, Lisa Dunn, at lisa@slac.stanford.edu for further information and guidance.
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Where can I find more information on cryo-EM data storage and transfer at S2C2?
Answer:
Please see our webpage on data access & transfer; as well as the website for the SLAC Shared Scientific Data Facility.
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Does Falcon 4 on TEM beta have an MTF file to use in Relion?
Answer:
Yes, we can send that to you. Contact your Scientific Point of Contact to request this file.
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What is the spherical aberration value for the Krios?
Answer:
Cs value is 2.7 mm.
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Does TEM Alpha support EER format?
Answer:
Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.
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Why is my CTF correction on all images above a certain value?
Answer:
Thick ice and particle distribution may affect the CTF value.
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Can you recover and return my grids with the shipping dewar?
Question details:
Answer:
Yes, we can. Please, ask your Scientific Point of Contact to do so prior to the conclusion of your microscope session. See our User Access page for more information.
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Can you store my unused grids for a later microscope session?
Answer:
Yes, we have dewar storage space dedicated to user cryo-specimens. Please, ask your Scientific Point of Contact to do so.
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Can EPU collect data on lacey grids?
Answer:
Yes, data can be collected from lacey carbon grids using EPU.
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How many images can we expect per hour - and therefore, per two-day microscope session?
Answer:
This depends on your data acquisition magnification, foil hole size, sample, and whether stage tilt or beam shift is used in data collection.
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