Frequently Asked Questions (FAQs)

  • When my project expires can I request an extension?

    Answer:

    Yes, upon submitting an extension form you may have a one-year extension.  Pls should contact our S2C2 User Administrator, Lisa Dunn, at lisa@slac.stanford.edu for further information and guidance.

  • Where can I find more information on cryo-EM data storage and transfer at S2C2​?

    Answer:

    Please see our webpage on data access & transfer; as well as the website for the SLAC Shared Scientific Data Facility.

  • Does Falcon 4 on TEM beta have an MTF file to use in Relion?

    Answer:

    Yes, we can send that to you. Contact your Scientific Point of Contact to request this file.

  • What is the spherical aberration value for the Krios?

    Answer:

    Cs value is 2.7 mm.

  • Does TEM Alpha support EER format?

    Answer:

    Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.

  • Why is my CTF correction on all images above a certain value?

    Answer:

    Thick ice and particle distribution may affect the CTF value.

  • Can you recover and return my grids with the shipping dewar?

    Question details:

    Answer:

    Yes, we can.  Please, ask your Scientific Point of Contact to do so prior to the conclusion of your microscope session.  See our User Access page for more information.

  • Can you store my unused grids for a later microscope session?

    Answer:

    Yes, we have dewar storage space dedicated to user cryo-specimens.  Please, ask your Scientific Point of Contact to do so.

  • Can EPU collect data on lacey grids?

    Answer:

    Yes, data can be collected from lacey carbon grids using EPU.

  • How many images can we expect per hour - and therefore, per two-day microscope session?

    Answer:

    This depends on your data acquisition magnification, foil hole size, sample, and whether stage tilt or beam shift is used in data collection.

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