Frequently Asked Questions (FAQs)

Does Falcon 4 on TEM beta have an MTF file to use in Relion?

Answer:

Yes, we can send that to you. Contact your Scientific Point of Contact to request this file.
What is the spherical aberration value for the Krios?

Answer:

Cs value is 2.7 mm.
Does TEM Alpha support EER format?

Answer:

Yes, we have added this format for efficient file storage with preservation of spatial and temporal resolution.
Why is my CTF correction on all images above a certain value?

Answer:

Thick ice and particle distribution may affect the CTF value.
Can EPU collect data on lacey grids?

Answer:

Yes, data can be collected from lacey carbon grids using EPU.
What is the nominal magnification for my requested pixel size?

Answer:

Nominal magnification tables for our microscopes can be found here. They are also posted at every TEM operation station.

Answer: