Workshop and Lecture Archive

2022 Workshop/Lectures

2021 Workshop/Lectures

2020 Workshop/Lectures

2019 Workshop/Lectures

 

2022

SSRL/LCLS Users' Meeting - CryoEM Workshop

Virtual Workshop | September 26, 2022 | Agenda
Workshop Presentations (not including River Xiao) (no PDFs of talks)
Speaker
Institution
Title
Monday, September 26, 2022
Peter Peters
Maastricht University
Sample preparation for Single Particle Analysis and cryo-EM tomography
Chu-Young Kim
 Yale University
Detection and repair of noncovalent DNA modifications
Chencheng Fan
Caltech
Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitope
Melani Ohi
University of Michigan
Structure and functional analysis of pathogenic T4SS using cryo-EM
River Xiao
University of Texas
Structural Studies of giant icosahedral eukaryotic dsDNA viruses
David Chmielewski
 
Chikungunya virus assembly and budding visualized in situ using cryoET

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S2C2 High Resolution CryoET Image Processing Virtual Workshop

Virtual Workshop | March 1 - 3, 2022 | Agenda

Workshop Materials: D. DeRosier's Fourier Transform Treastise Booklet, T. Goddard's Materials: Zip Folder, S. Ludtke's Tutorial: Zip Folder

Speaker Institution Title Slide Deck Recording
Tuesday, March 1, 2022
 David DeRosier   Brandeis University  EM Images:what you want vs what you get and why   SD1  R1
 Frederick Sigworth   Yale University Basics of Image Processing: from the data you have to the structures you'd like  SD1 1
 Thomas Goddard  UC San Francisco Visualizing proteasomes in tomograms with ChimeraX SD1 R1
Wednesday, March 2, 2022
 Steven Ludtke  Baylor College of Medicine   CryoET processing toward atomic resolution (EMAN2) SD1  SD2 R1, R2, R3
Thursday, March 3, 2022
 Dimitry Tegunov  Genentech Using the WARP-RELION-M pipeline to obtain high-resolution structures from tilt series  SD1 R1,R2,R3
 

 

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2021

SSRL/LCLS Users Meeting:  CryoEM of Macromolecules and Cells  Workshop

Virtual Workshop | September 21, 2021 | Agenda
Speaker Institution Title Slide Deck Recording
Alfonso Mondragon      Northwestern University Structural Studies of a type III-A CRISPR effector compleex SD1 -
Seyed Torabi Yale University Integration of biochemical and structural data to investigate stabilizing triplex-forming elements in RNA SD1 R1
Kalli Kappel MIT Broad Institute Accelerating RNA structure determination with cryo-EM and DRRAFTER computational modeling SD1 R1
Zhaoming Su Sichuan University Cryo-EM reveals multiple states in splicing reaction of Tetrahymena intron SD1 R1
Kaiming Zhang University of Science and Technology of China Determination of small RNA-only 3D structures by Cryo-EM SD1 R1

 

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S2C2 CryoEM Map Modeling and Validation Workshop 

Virtual Workshop | September 8-10, 2021 | Agenda
Workshop Materials:  Computer Setup & Instructions; Q&A Day 1 Day 2 Day 3
Speaker
Institution
Title
Slide Deck
Recording
Wednesday, September 8, 2021
Christopher Schlicksup
Lawrence Berkeley National Laboratory
Phenix tools for improving maps, and building, refining, and validating models
Tom Goddard
UC San Francisco
Using AlphaFold predicted structures in ChimeraX for cryoEM modeling
Grigore Pintilie
Stanford University
Model fitting and assessment in CryoEM maps with Segger, Segfit, and Q-scores
Jasmine Young
Justin Flatt
Rutgers University
Deposition, Validation, and Biocuration at the wwPDB
Thursday, September 9, 2021
Paul Emsley MRC Laboratory of Molecular Biology Coot Tools for Cryo-EM Model-building, Refinement, Validation, and Analysis SD1 R1
Tristan Croll Cambridge University Interactive physics-based model building with ISOLDE SD1 R1

Maximilian Edich

Jola Mirecka

Universität Hamburg

STFC UKRI

Secondary SD1 R1
Grzegorz Chojnowski EMBL, Hamburg Macromolecular models automatically built into cryo-EM maps and how to use them SD1 R1
Jane Richardson Duke University CryoEM model validation, to avoid or correct errors at various resolutions SD1 R1
Friday, September 10, 2021
Tristan Cragnolini Birbeck College Improving and validating map/model fits with TEMPy SD1 R1
Brinda Vallat Rutgers University Virtual Torsion Plots to Validate Coarse RNA Conformation SD1 R1
Andriy Kryshtafovych UC Davis Model comparisons SD1 R1
Ardan Patwardhan EMBL-EBI Cryo-EM validation from the perspective of the EMDB and EMPIAR archives SD1 R1
Abhishek Singharoy Arizona State University Molecular dynamics flexible fitting extended: structures, ensembles and free energy of proteins and small molecules

 

SD1

SD2

R1

 

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S2C2 CryoEM Specimen Preparation and Data Collection Workshop

Virtual Workshop | March 3-4, 2021 | Agenda
Speaker
Institution
Title
Slide Deck
Recording
Wednesday, March 3, 2021
Peter J. Peters          
(M4i) Maastricht University & CryoSol-World
Understanding the invisible hands of sample preparation for cryo-EM
Benjamin Bammes
 Direct Electron, LP
Optimizing Direct Detector Design for a Range of Cryo-EM Techniques
Jeffrey Lengyel
Thermo Fisher
The latest generation technology for Cryo-EM allowing for unparalleled scientific breakthroughs
Thursday, March 4, 2021
Christopher Russo
LMB, MRC, Cambridge UK
Principles of information loss in cryoEM and how eliminating specimen movement can improve cryomicrographs
R1,R2,R3
Sahil Gulati
Ametek
Advances in direct electron detection: getting the most out of your microscope
Yee Ting Li
SLAC National Accelerator
Data Management at S2C2 
Patrick Mitchell
SLAC National Accelerator
S2Cdata collection
All
 
Final Q&A
-
1,2,3

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2020

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S2CCryoEM CCP-EM Modeling Workshop

Virtual Workshop | November 10-13, 2020 | Agenda
Workshop Materials: computing instructions
Speaker
Institution
Title
Slide Deck
Recording
Tuesday, November 10, 2020
Colin Palmer
CCP-EM
Lecture: Intro to workshop and CCP-EM
Kevin Cowtan
University of York
Lecture: Automated model building with Buccaneer & Nautilus
Kevin Cowtan
University of York
Tutorial: Buccaneer
-
Paul Emsley
MRC, LMB
Lecture: Cryo-EM model building with modern Coot
Paul Emsley
MRC, LMB
Tutorial: Coot (part 1)
-
Wednesday, November 11, 2020
Paul Emsley
MRC, LMB
Tutorial: Coot (part 2)
-
Maya Topf
ISMB, Birkbeck
Lecture: Flexible fitting of Atomic Models
Tristan Cragnolini
ISMB, Birkbeck
Tutorial: Flex-EM (part 1)
-
Aaron Sweeney
Univ. College London
Tutorial: Flex-EM (part 2)
-
Andrea Thorn
University of Würzburg
Lecture: Finding secondary structure in cryo-EM maps: HARUSPEX
Thursday, November 12, 2020
Rob Nicholls
MRC, LMB
Lecture Refinement against cryo-EM Maps
Rob Nicholls
MRC, LMB
Tutorial: Refmac (part 1)
-
Rob Nicholls
MRC, LMB
Tutorial: Refmac (part 2)
-
Ardan Patwardhan
EMBL-EBI
Lecture: PDB, EMDB, & Empiar: Public archiving of cryo-EM data
-
Arjen Jakobi
TU Delft
Lecture: Map sharpening tools for cryo-EM density maps
Arjen Jakobi
TU Delft
Tutorial: LocScale
-
Friday, November 13, 2020
Agnel Joseph
CCP-EM
Lecture: Validation of cryo-EM maps and models
Agnel Joseph
CCP-EM
Lecture: Model Validation
-
Agnel Joseph
CCP-EM
Tutorial: Model Validation
-
All Speakers
 
General Q&A Session
-
 

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SSRL/LCLS Users' Meeting: Cryo-EM of COVID-related Macromolecules

Virtual Workshop | September 28, 2020 | Agenda
Speaker
Institution
Title
Slide Deck
Recording
David Veesler
University of Washington
Structural Studies of the SARS-CoV Spike Glycoprotein
-
David Stuart
Oxford University, Diamond Light Source
The use of cryoEM structural information to advance SARS2-CoV-2 biology and therapy
Goran Kokic
Max Planck Institute, Göttingen
Structure of replicating SARS-COV-2 polymerase
Erica O. Saphire Adrian Enriquez
La Jolla Institute
Antibodies against emerging viral disease
-
Rhiju Das
Ivan Zheludev
Stanford University
CryoEM of previously unsolvable RNA structures including coronavirus genome elements
Stephan Wilkens
SUNY Upstate Medical University
Targeting Vacuolar H+-ATPase for Antiviral Therapy
R1, R2, R3

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Fundamentals of Single Particle CryoEM Workshop

Virtual Workshop | August 11 - 12, 2020 | Agenda                      
Speaker
Institution
Title
Slide Deck
Recording
Tuesday, August 11, 2020
Mark Herzik
UC San Diego
Sample preparation for cryo-EM
David DeRosier
Brandeis University
Image contrast theory and reconstruction
Alexis Rohou
Genentech
Principles of image processing
Andres Leschziner
UC San Diego
Determining structures of challenging samples
Wednesday, August 12, 2020
Dmitry Lyumkis
Salk Institute
Dealing with resolution anisotropyand considerations for high resolution structure determination
R1, R2, R3
Wah Chiu
Stanford/SLAC
Atomic modeling and the importance of validation
Ali Punjani
Structural Biotechnology
CryoSPARC demonstration/tutorial (see June Workshop for slides)
-
R1, R2, R3, R4

 

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S2C2 CryoEM Image Processing Workshop: cryoSPARC

Virtual Workshop | June 10 - 12, 2020 | Agenda
Workshop Materials: user interface overview, cryoSPARC instructions Day 1 / Day 2, Q&A Day 1, Day 2, Day 3
Speaker
Institution
Title
Slide Deck
Recording
Wednesday, June 10, 2020
David DeRosier
Brandeis University
If only...
R1, R2, R3
Ali Punjabi
Structural Biotechnology
CryoSPARC (AM) 06/10/20 User interface overview/Day 1 CryoSPARC Instructions
R1, R2, R3, R4, R5, R6
Ali Punjabi
Structural Biotechnology
CryoSPARC (PM) 06/10/20
SD1 
R7, R8, R9, R10, R11, R12
Thursday, June 11, 2020
Ali Punjabi
Structural Biotechnology
CryoSPARC (AM) 06/11/20
R1, R2, R3, R4, R5, R6, R7, R8
Ali Punjabi
Structural Biotechnology
CryoSPARC (PM) 06/11/20
Stephan Arulthasan
Structural Biotechnology
CryoSPARC architecture & management
Ali Punjabi
Structural Biotechnology
CryoSPARC (PM) 06/11/20
-
13, 14, 15, 16
Friday, June 12, 2020
Greg Pintilie
Stanford University
CryoEM map resolution assessment, validation, visualization and segmentation
R1, R2, R3
Cathy Lawson
Rutgers University
EMDataResource: structure data archiving, validation challenges

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Basics of Sample Preparations & Detection Technologies for Cryo-Electron Microscopy

Virtual Lecture | May 29, 2020
Speaker
Institution
Title
Slide Deck
Recording
Yifan Cheng
HHMI UC San Francisco
 Basics of sample preparation
-
-
Christopher Booth
Amtek
Detection technologies for cryo-electron microscopy
R1, R2, R3, R4, R5, R6

 

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General Principles of Phase Contrast and Phase Plates & Single-Particle Grid Preparation

Virtual Lectures | April 20, 2020
Speaker Institution Title Slide Deck Recording
Glaeser, Robert Lawrence Berkeley National Lab General principles of phase contrast and pase plates SD1 R1
Glaeser, Robert Lawrence Berkeley National Lab Single-particle grid preparation SD1 R1

 

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Specimen Prep and Data Collection Workshop

In-Person Workshop | January 15 - 17, 2020 | Cancelled by COVID-19

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S2C2 CryoEM Modeling Workshop: ChimeraX & Phenix

In-Person Workshop | January 15 - 17, 2020 | Agenda  
Speaker
Institution
Title
Slide Deck
Recording
Wednesday, January 15, 2020
Tom Goddard 
Elaine Meng
Eric Pettersen
UC San Francisco
ChimeraX-Visualization of cryoEM maps and models
-
Greg Pintilie
Stanford University
Segmentation, rigid body and flexible fitting
-
Tom Goddard
Elaine Meng
Eric Pettersen
UC San Francisco
Refinement of cryoEM maps with Isolde
-
Tom Goddard
UC San Francisco
Publication-quality images and movies
-
Thursday, January 16, 2020
Paul Adams
Lawrence Berkeley National Lab
Phenix introduction (LBNL videos are all included on this video)
Paul Adams
Lawrence Berkeley National Lab
Atomic models from cryo-EM data
-
Pavel Afonine
Lawrence Berkeley National Lab
Structure Refinement
-
Paul Adams
Lawrence Berkeley National Lab
Model validation for cryo-EM
-
Pavel Afonine
Lawrence Berkeley National Lab
Structure refinement and validation practical
-
Nigel Moriarty
Lawrence Berkeley National Lab
Ligands
-
Friday, January 17, 2020
Greg Pintilie
Stanford University
Assessment of high resolution cryoEM features
Helen Berman
Rutgers University
Validation of biological structures
-
-
Cathy Lawson
Rutgers University
EM structure archives, validation and challenges (included with Greg Pintilie's video)
-

 

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2019

S2C2 CryoEM Image Processing Workshop: SCIPION

Workshop Material: agenda

Speaker Institution Title Slide Deck Recording
Monday, November 18, 2019
David DeRosier Brandeis University General principles of cryoEM 3D reconstructions SD1 R1, R2, R3
Jose Maria Carazo CNB-CSIC Basic image processing for EM SD1, SD2 R1, R2, R3
Carlos Sanchez-Sorzano CNB-CSIC Image processing principles SD1 R1R2R3R4, R5
Tuesday, November 19, 2019
Yiorgio Skiniotis Stanford University Dealing with heterogeneity in single-particle data/examples SD1 -
Wednesday, November 20, 2019
Greg Pintilie Stanford University Model building and map validation - -

 

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S2C2 CryoEM Training for Beginners Workshop

Workshop Materials: agenda, Prof. Grant Jensen Videos)

Speaker Institution Title Slide Deck Recording
Wednesday, September 11, 2019
Juergen Plitzko Max Planck Institute EM basics SD1 -
Yiorgio Skiniotis Stanford University Freezing basics SD1 -
Thursday, September 12, 2019
Christopher Booth Gatan Camera SD1 -
Yee Ting Li SLAC National Accelerator Lab eLogbook and data transfer - -
Robert Glaeser Lawrence Berkeley National Lab Radiation damage SD1 -
Friday, September 13, 2019
Zoltan Metlagel FEI EPU and phase plate - -
Corey Hecksel Stanford University Data collection (serial EM) SD1 -

 

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S2CCryoEM Modeling Workshop: ChimeraX & Phenix 

Workshop Materials: agenda, properly weighted map t20s, model validation tutorial/model optimization tutorial

Speaker Institution Title Slide Deck Recording
Wednesday, July 10, 2019
Tom Goddard                  Elaine Meng
Eric Pettersen
UC San Francisco ChimeraX - visualization of cryoEM maps and models SD1 -
Greg Pintilie Stanford University Rigid body and flexible fitting SD1 -
Thursday, July 11, 2019
Paul Adams Lawrence Berkeley National Lab (LBNL) Phenix introduction SD1 -
Paul Adams LBNL Automated model docking and building SD1 -
Pavel Afonine LBNL Phenix tools for cryoEM: refinement and validation SD1 -
Paul Adams LBNL Model validation for cryo-EM SD1 -
Nigel Moriarty LBNL Restraints in Phenix SD1 -
Friday, July 12, 2019
Corey Hyrc Baylor College of Medicine Accurate representaiton of cryoEM structures SD1 -
Greg Pintilie Stanford University Assessment of high resolution of cryoEM features SD1 -
Michael Robertson Stanford University CryoEM structure and ligand modeling - -
Cathy Lawson Rutgers University CryoEM archives, challenges and validation SD1 -

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S2C2 CryoEM Image Processing Workshop:  SCIPION

In-Person Workshop | November 18 - 20, 2019 | Agenda
Speaker
Institution
Title
Slide Deck
Recording
Monday, March 11, 2019
David DeRosier
Brandeis University
General principles of cryoEM image reconstruction
-
Yee Ting Li
SLAC National Accelerator Lab
Data acquisition and pre-processing software logging on and computer basics
-
Cornelius Gati
SLAC
Image processing with Relion - introduction
-
-
Tuesday, March 12, 2019
Cornelius Gati
SLAC
Practical 2 & 3
-
-
Wednesday, March 13, 2019
Greg Pintilie / Wah Chiu
Stanford University
Map visualization and validation
_
Cathy Lawson
Rutgers University
Cryo-EM map challenge, map deposition and archiving
-
Yiorgio Skiniotis 
Stanford University
Dealing with heterogeneity in single-particle cryoEM data
-
Cornelius Gati
SLAC
Different image processing software packages
-
-

 

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S2C2 CryoEM Training for Beginners Workshop

In-Person Workshop | September 11 - 13, 2019 | Agenda   (no recordings for this workshop)
Speaker
Institution
Title
Slide Deck
Recording
Wednesday, September 11, 2019
Juergen Plitzko
Max Planck Institute
EM Basics
SD1
_
Yiorgo Skiniotis
Stanford
Freezing Basics
SD1
-
Hands On
Practical
Corey Hecksel, Megan Mayer, Zoltan Metlagel, Nancy Rizzo
SD1
-
Thursday, September 12, 2019
Chris Booth
Gatan
Camera
SD1
-
Yee-Ting Li
SLAC
eLogbook and data transfer
SD1
-
Robert Glaeser
Lawrence Berkeley National Lab
Radiation Damage
SD1
-
Hands On
Practical
Corey Hecksel, Megan Mayer, Zoltan Metlagel, Nancy Rizzo
SD1
-
Friday, September, 13, 2019
Zoltan Metlagel
Thermofisher
EPU and Phase Plate
SD1
-
Corey Hecksel
SLAC
Data Collection (Serial EM)
SD1
-
Hands On
Practical
Corey Hecksel, Megan Mayer, Zoltan Metlagel, Nancy Rizzo
SD1
-

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S2C2 Modeling Workshop: ChimeraX & Phenix

Speaker
Institution
Title
Slide Deck
Recording
Wednesday, July 10, 2019
Tom Ferrin/ Tom Goddard
UCSF
ChimeraX - Visualization of CryoEM maps and Models
SD1
-
Greg Pintilie
Stanford
Rigid Body and Flexible Fitting
SD1
-
Michael Schmid
SLAC
Hands on VR to Visualize CryoEM Structures
SD1
-
Thursday, July 11, 2019
Paul Adams
Lawrence Berkeley Lab
Phenix Intro/Automated Model Docking and Building
SD1
-
Pavel Afonine
Lawrence Berkeley Lab
Structure Refinement
SD1
-
Dorothee Liebschner
Lawrence Berkeley Lab
Validation and Refinement
SD1
-
Paul Adams
Lawrence Berkeley Lab
Intro to Phenix Cryo-EM Tools Practical/Automated Model Building
SD1
-
Pavel Afonine
Lawrence Berkeley Lab
Structure Refinement Practical
SD1
-
Dorothee Liebschner
Lawrence Berkeley Lab
Validation and Refinement Practical
SD1
-
Friday, July 12, 2019
Corey Hryc
 
Accurate Representation of CryoEM Structures
SD1
-
Greg Pintilie
Stanford
Assessment of High Resolution CryoEM Features
SD1
-
Mike Robertson
Stanford
CryoEM Structure Ligand Modeling
SD1
-
Cathy Lawson
Rutgers
CryoEM Structural Validation
SD1
-
 
 
Practical with Apoferritin or other Molecules
SD1
-

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S2C2 Cryo-EM Image Processing Workshop

Speaker
Institution
Title
Slide Deck
Recording
Monday, March 11, 2019
David DeRosier
Brandeis University
General Principles of CryoEM Image Reconstruction
SD1
-
Yee-Ting Li
SLAC
Data Acquistion and pre-processing software, logging on and computer basics
SD1
-
Cornelius Gati
SLAC
Image Processing with Relion Intro/Practical 1: Images, MotionCor, CTF
SD1
-
Tuesday, March 12, 2019
Cornelius Gati
SLAC
Practical 2: 2D/3D Classification/Practical 3: 3D Refinement & Map Improvement
SD1
-
Wednesday, March 13, 2019
Greg Pintilie/Wah Chiu
SLAC
Map Visualization and Validation
SD1
-
Cathy Lawson Rutgers
Map Challenge and Map Deposition and Archival
SD1
-
Georgios Skiniotis
Stanford
Dealing with Heterogeneity in single-particle cryoEM data
SD1
-
Kaiming Zhang
Stanford
Image Processing of Heterogeneous Particle Images
SD1
-
Bart Buijsse
ThermoFisher
Update on Volta Phase Plate
SD1
-

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S2C2 Cryo-EM Training for Beginners Workshop

Speaker
Institution
Title
Slide Deck
Recording
Tuesday, January 22, 2019
Yiorgio Skiniotis
Stanford University
Cryo-specimen preparation
-
Wah Chiu
Stanford/SLAC
Radiation damage in cryoEM
-
Christopher Booth
Gatan
Detection technologies for cryo-electron microscopy
-
Wednesday, January 23, 2019
Juergen Plitzko
Max Planck Institute
TEM basics and column alignment
-
David Bushnell
Stanford/SLAC
Low dose imaging via serial EM and EPU
-
Wah Chiu
Stanford/SLAC
Image contrast theory in CryoEM
-
Thursday, January 24, 2019
John Spear
Thermofisher
Krios and Talos alignment
-
Yee Ting Li
SLAC
eLogbook, data collection monitoring and
pre-processing
-

Last Updated

Mon, 10/24/2022 - 15:19