This page details the information you will be asked to provide when applying to the project-tailored training program through the User Portal.
- Project Title (80 characters or less)
- Project Type (i.e., Data Collection Service, Exploratory Freezing & Screening, Project-tailored training, or Comprehensive Training)
- Project Abstract (150 words or less)
- Proposed Length of Training (pick one)
- • 1 week
- • 3 weeks
- • 5 week
- • 2 weeks
- • 4 weeks
- • 6 weeks
- Remote or In-person Training (pick one)
• Remote Training • In-person Training at SLAC
- CryoEM Workflows (pick all that apply)
• cryoEM Single Particle Analysis (cryoSPA)
• cryogenic electron tomography (cryoET)
• cryogenic electron diffraction (cryoED)
- Requested Training(s) (pick all that apply) *In-person training preferred
• Basic cryoTEM operation* • Cryo-specimen screening
• Cryo-specimen preparation* • Data collection & preprocessing
- ⋅Leica EM Plunge Freezer
- ⋅Vitrojet (coming soon)
- ⋅Chameleon (coming soon)
Project Documents (pdfs)
Training & Experience Document (1,000 words or less)
Goals & Motivation (150 words or less): State the specific goals for your training project and the motivation for seeking project-tailored training.
Current Research (200 words or less): Describe the trainee's current research projects and justify the need to include cryoEM in your future research.
Institutional Impact (150 words or less): Describe how this training will benefit others beyond yourself, i.e., your research group, department, and the wider research community at your institution. How will your experiences be shared with others?
Cryogenic Electron Microscopy Experience (200 words or less): Describe the trainee's experience with cryogenic electron microscopy (cryoEM), specifically, including the location, previous training, and the specimen preparation and microscopy methods employed (e.g., cryogenic TEM, cryogenic SEM, cryogenic electron tomography (cryoET), sincle particle analysis (SPA), cryogenic focus ion beam milling, cryogenic microtomy, cryogenic electropolishing, plunge vitrification, and jet vitrification.)
Other Electron Microscopy Experience (200 words or less): Describe the trainee's experience with electron microscopy, generally, incluidng location, previous training, and the methods performed (e.g., transmission electron microscopy (TEM), bright field TEM (BF-TEM, dark field TEM (DF-TEM), high resolution TEM (HRTEM, energy filtered TEM (EFTEM), electron diffraction (ED), scanning transmissaion electron microscopy (STEM), bright field STEM (BF-STEM), dark field STEM (DF-STEM), high-angle annular dark field STEM (HAADF-STEM), scanning electron microscopy (SEM), secondary electron imaging SEM (SE-SEM), back-scattered electron imaging SEM (BSE-SEM), analytical electron microscopy (AEM), energy-dispersive x-ray spectroscopy (EDS), electron energy loss spectroscopy (EELS), electron spectroscopic imaging (ESI), freeze-fracture electron microscopy, negative-stain TEM, in-situ TEM, or reflection electron microscopy (REM).)
Operational Experience (100 words or less): List the electron microscopes and specimen preparation instruments the trainee has operational experience on including manufacture and model (e.g., JEOL Cryo ARM 200 TEM, JEOL JEM-2100Plus TEM, ThermoFisher Talos Arctica TEM, ThermoFisher Krios G2 TEM, TFS Vitrobot Mark II, Leica EM GP2 Automatic Plunge Freezer, etc...)
Project Description Document (1,000 words or less)
Aims & Impact (150 words or less): State the specific objectives or your project, include the scientific and technological importance of your project.
Feasibility & Data (300 words or less): Provide information and preliminary data for the samples associated with this proposal that impact feasibility of cryoEM studies (e.g., molecular weight stability, homogeneity, sufficient concentrations, etc.). Reference all figures uploaded in the Preliminary Data Document and provide a brief description, as appropriate.
Proposed Experiments (150 words or less): Describe the work to be conducted at the national center during the awarded project period. For each aim and/or sample under investigation, provide a detailed description of the experiment(s) to be performed and expected outcomes. Strength of justification can affect the overall science and resource scores.
Goals & Expectations (200 words or less): Describe the goals for this project. This section should also include a justification for specific instruments requested, if any, and for the estimated allocation of time needed for each experiment.
Expertise & Resources (200 words or less): Describe the team's expertise in cryoEM sample preparation, microscope operation, and data interpretation. Please state the available cryoEM and/or computational resources that your team has access to.
Preliminary Data Document (i.e., Figures/Preliminary Results): Scientific figures with captions that are referred to within the Feasibility & Data section of the Project Description Document. Experimental preliminary data may include SDS-PAGE gels, SEC traces, negative-stain electron micrographs, preliminary cryo-EM screening, 2D class averages, lower resolution 3D reconstruction, etc...
Trainee Biosketch or Curriculum Vitae Document
PI Biosketch or Curriculum Vitae Document (optional)
Provide a description of your samples including the type(s), source organism(s) and associated hazards such as toxicity and biohazard safety level (risk group).
Upload material approval documents from your institution (e.g., IBC documents, MSDS, approved SOP/protocols). If not presently available, this documentation must be provided with the microscope time request later in this process.
Identify the members of your research team. The Principal Investigator is the person responsible for the overall research in this project. The Trainee is also able to fulfill the responsibilities of submitting microscope time requests and corresponding with S2C2 staff. The person submitting the proposal is automatically made the trainee, but this may be changed to another team member at the time of application.
Principle Investigator: The Principal Investigator (PI) is the lead scientist for the overall research project. PIs are generally the "research group leader" or "head of the laboratory" who whare authority and responsibility for leading and directing the research, intellectually and logistically. PIs typically conceive of the investigations and are accountable to their organizations and funding agencies. Unless authority is delegated to a Lead Contact the PI takes responsibility for the coordination of proposal scheduling, access to the facility by proposal collaborators, and oversight of the experiments conducted at the facility.
Collaborators: Individuals who are invited to participate in the proposed research. Collaborators are regarded as Co-proposers and participate in the reesearch in various ways including sample preparation, theory, related experimentation, data collection, data reduction, data analysis, presentation of results, and/or publications.
Collaborating PI(s): Collaborating PI(s) are involved in the intellectual aspects of the project and may contribute to the overall data collection, processing strategy, and funding to achieve the project aims.
Trainee: The Trainee will share the PI's responsibility for th econduct of the proposed experiments, including coordination of training scheduling, access to the facility, and oversight of the experiments conducted at the facility.
To enter any individual, start with their last name or email address to access and select names already in our database. If no matching record is found, enter a name and email address. They will be invited to register in the SLAC User Portal at the time the proposal is accepted.
Provide the funding source, grant number, and grant title supporting biological sample procuement or production, trainee transportation, local room and board for onsite trainee, and shipping of samples (and dewar return shipping).
List publications, patents, and major awards that resulted from previous CryoEM experience.